Clonality | Host Species | Isotype
HIS.H8
/ EH158 | Mouse | IgG2b
Immunogen
HHHHHH
(6x His) synthetic peptide conjugated to KLH
Specificity
Recognizes
the epitope of 6x His-tags encoded by many commercially available vectors,
regardless of the tag's location in the fusion protein sequences (i.e.: reacts
with N-terminal, C-terminal or internal 6x His-tags)
Purification
Protein
A affinity chromatography from mouse ascites fluid
Applications
Dot,
ELISA, IP, IS, WB
Sensitivity
WB
(with ECL): 1-2ng of His-tagged fusion proteins
Form
1mg/mL
in 10mM PBS, pH 7.2, with 0.05% NaN3
Working Conditions
WB
(with ECL): 1:1000-3000 dilution (incubate for one hour at room temperature)
IS:
1:500-2000 dilution
For
best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine
optimal working dilution by titration test
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