| Control Cell Lysate - Hela Cell Extract- CG023 |
Product Name HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Product type Lysates
Description HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Tested applications Usefull as control for western blot
Recommended amounts 10-50 ug per gel lane
Relevance
HeLa cells are human epithelial cells from a fatal cervical carcinoma. The cell line was derived from cervical cancer cells taken from Henrietta Lacks, in 1951. Horizontal gene transfer from human papillomavirus 18 (HPV18) to human cervical cells created the HeLa genome which is different from either parent genome in various ways including its number of chromosomes. HeLa cells have a modal chromosome number of 82, with 4 copies of chromosome 12 and 3 copies of chromosomes 6, 8, and 17.
HeLa cells are adherent cells (they stick to surfaces) and maintain contact inhibition in vitro.
Quality control:
1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot.
2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
Storage buffer : SDS loading buffer
Concentration
5 mg/ml
Storage instructions
Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
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