| Anti-Dicer Antibody 13D6R - Biomarker in Melanoma |
In recent work, CLONEGENE's antibodies have been used to measure the expression of Dicer and identify Melanoma subtypes of clinical interest (click on picture below to access the original paper ). Dicer is an endoribonuclease in the RNase III family that cleaves double-stranded RNA (dsRNA) and pre-microRNA (miRNA) into short double-stranded RNA fragments called small interfering RNA (siRNA) about 20-25 nucleotides long, usually with a two-base overhang on the 3' end. Dicer contains two RNase III domains and one PAZ domain; the distance between these two regions of the molecule is determined by the length and angle of the connector helix and determines the length of the siRNAs it produces. Dicer catalyzes the first step in the RNA interference pathway and initiates formation of the RNA-induced silencing complex (RISC), whose catalytic component argonaute is an endonuclease capable of degrading messenger RNA (mRNA) whose sequence is complementary to that of the siRNA guide strand. Thus Dicer plays a pivotal role in the cell by regulating the production of microRNAs.
CLONEGENE has developed a rabbit antibody (R13D6) against a highly conserved peptide in human Dicer. This antibody reacts with human, mouse, rat, cow, dog and lizard Dicer . The sequence of the peptide is confidential but we are happy to advise you if this antibody will work on Dicer from your species of interest. Working conditions are 1/5,000 for IHC and 1/2000 for western blot making this a very economical purchase. R13D6 was generated against the same synthetic peptide that was used to generate our anti-Dicer 13D6 and 4A6 mouse monoclonals. Indeed, R13D6 is now the reagent that we suggest for IHC on FFPE sections. R13D6 is provided as an affinity-purified IgG (1mg/ml).
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